cd133 micro beads  (Miltenyi Biotec)

Journal: Indian Journal of Ophthalmology

Article Title: A short-term chick embryo in vivo xenograft model to study retinoblastoma cancer stem cells

doi: 10.4103/ijo.IJO_2348_21

Figure Lengend Snippet: Flow cytometry analysis of CD133 expression in Rb Y79 cell line. (a) Scatter plot of Y79 cells with gating around the live population. (b and c) Doublet discrimination plots. (d) CD133-APC expression. (e) Analysis of the subpopulations

Article Snippet: Cultured Y79 cells and eGFP Y79 cells were sorted using CD133 micro bead kit according to manufacturer’s protocol (MiltenyiBiotec Inc., Auburn, CA) as described previously.

Techniques: Flow Cytometry, Expressing

Journal: Indian Journal of Ophthalmology

Article Title: A short-term chick embryo in vivo xenograft model to study retinoblastoma cancer stem cells

doi: 10.4103/ijo.IJO_2348_21

Figure Lengend Snippet: CD133 expression of Rb Y79 cells in different passages

Article Snippet: Cultured Y79 cells and eGFP Y79 cells were sorted using CD133 micro bead kit according to manufacturer’s protocol (MiltenyiBiotec Inc., Auburn, CA) as described previously.

Techniques: Expressing

Journal: Indian Journal of Ophthalmology

Article Title: A short-term chick embryo in vivo xenograft model to study retinoblastoma cancer stem cells

doi: 10.4103/ijo.IJO_2348_21

Figure Lengend Snippet: Assessment of tumor nodules formation on CAM and quantitative measurement of tumor nodules Tumor nodules of (a) CM-Dil CD133 - transplanted cells, (b) CM-Dil CD133 + transplanted cells, (c) eGFP Y79 CD133 - transplanted cells, and (d) eGFP Y79 CD133 + transplanted cells. (e) Quantitative measurement of tumor volume of eGFP Y79 transplanted cells (CD133 - vs CD133 + , **P = 0.0014)

Article Snippet: Cultured Y79 cells and eGFP Y79 cells were sorted using CD133 micro bead kit according to manufacturer’s protocol (MiltenyiBiotec Inc., Auburn, CA) as described previously.

Techniques:

Journal: Indian Journal of Ophthalmology

Article Title: A short-term chick embryo in vivo xenograft model to study retinoblastoma cancer stem cells

doi: 10.4103/ijo.IJO_2348_21

Figure Lengend Snippet: Confocal imaging and analysis of tumors formed on the CAM layer Representative confocal images of CAM nodules formed by (a) CM-Dil Y79 CD133 - cells, (b) CM-Dil Y79 CD133 + cells, (d) eGFP Y79 CD133 - cells, (e) eGFP Y79 CD133 + cells. Quantification of fluorescence showed that the CD133 - cells of CM-Dil Y79 (c) and eGFP Y79 (f) displayed an increased localization compared to their CD133 + counterparts (****P < 0.0001)

Article Snippet: Cultured Y79 cells and eGFP Y79 cells were sorted using CD133 micro bead kit according to manufacturer’s protocol (MiltenyiBiotec Inc., Auburn, CA) as described previously.

Techniques: Imaging, Fluorescence

Journal: Indian Journal of Ophthalmology

Article Title: A short-term chick embryo in vivo xenograft model to study retinoblastoma cancer stem cells

doi: 10.4103/ijo.IJO_2348_21

Figure Lengend Snippet: Evidence of Rb spontaneous metastasis in different parts of chick embryo analyzed using in vivo IVIS spectral imaging. Representative image of in vivo fluorescence observed in chick embryo transplanted with (a) CM-Dil Y79 CD133 - cells, (b) CM-Dil Y79 CD133 + cells, (d) eGFP Y79 CD133 - cells, and (e) eGFP Y79 CD133 + cells. Quantification of fluorescence intensity within the chick embryo revealed that CD133 - cells had increased spontaneous metastasis when compared to CD133 + cells (**P = 0.0077, 0.0017)

Article Snippet: Cultured Y79 cells and eGFP Y79 cells were sorted using CD133 micro bead kit according to manufacturer’s protocol (MiltenyiBiotec Inc., Auburn, CA) as described previously.

Techniques: In Vivo, Imaging, Fluorescence

Journal: Indian Journal of Ophthalmology

Article Title: A short-term chick embryo in vivo xenograft model to study retinoblastoma cancer stem cells

doi: 10.4103/ijo.IJO_2348_21

Figure Lengend Snippet: Histopathology of Rb Y79 CD133 - and CD133 + xenografts on the CAM layer (a) Histopathology of CAM transplanted with Y79 CD133 - (CSCs) showed larger cells with increased N/C ratio. (b) The proliferating CD133 - cells showed immunoreactivity with Ki-67. (c) Histopathology of CAM transplanted with Y79 CD133 + (non-CSCs) showed multiple foci of tumor infiltrates, edema, and inflammation within the CAM stroma and (d) The Y79 CD133 + cells were not reactive to the cellular proliferative marker, Ki-67

Article Snippet: Cultured Y79 cells and eGFP Y79 cells were sorted using CD133 micro bead kit according to manufacturer’s protocol (MiltenyiBiotec Inc., Auburn, CA) as described previously.

Techniques: Histopathology, Marker

Journal: Indian Journal of Ophthalmology

Article Title: A short-term chick embryo in vivo xenograft model to study retinoblastoma cancer stem cells

doi: 10.4103/ijo.IJO_2348_21

Figure Lengend Snippet: Histopathological evidence of metastasis into the embryonic organs. The embryos transplanted with Y79 CD133 - cells show tumor cells within the (a) blood vessels of the liver (vascular emboli), (b) brain, and (c) eye. Immunohistochemical analysis of Ki-67 marker revealed positive cells within the (d) liver, (e) brain, and (f) eye

Article Snippet: Cultured Y79 cells and eGFP Y79 cells were sorted using CD133 micro bead kit according to manufacturer’s protocol (MiltenyiBiotec Inc., Auburn, CA) as described previously.

Techniques: Immunohistochemical staining, Marker